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Table 3 Antibodies used in western blotting and ChIP analyses

From: Epigenetic regulation of histone modifications and Gata6 gene expression induced by maternal diet in mouse embryoid bodies in a model of developmental programming

Antibody

Source

Code/dilution

Technique

Gata6

R&D Systems

AF1700, 1:100

westerns

Dab-2

BD Biosciences

610465, 1:1,000

westerns

α-tubulin

Cell Signaling

2144, 1:500

westerns

H4Ac

Millipore

06-866

ChIP

H3Ac

Millipore

06-599

ChIP

H3K4Me3

Actif Motif

339916

ChIP

H3K4Me2

Actif Motif

339914

ChIP

H3K9Me3

Abcam

ab8898

ChIP

H3K27Me3

Abcam

ab6002

ChIP

RNA Polymerase II

Abcam

ab5408

ChIP