Table 1 Primers used in qRT-PCR and ChIP Q-PCR
Primer name | Forward primer (5′-3′) | Reverse perimer (5′-3′) | Assay |
|---|---|---|---|
Gata4 P1 | gggctggtggaggttctc | tcagtgcctagagacgcaag | ChIP Q-PCR |
Gata4 P3 | gccattctctgcattcatcc | tcgctgagcatcaaggaac | |
Gata4 P5 | tctgagaggagccgataacc | gaactaggcgacctctgtgc | |
Gata6 P1 | catttggagggagcgactaa | tccaaggacgctagtttggt | |
Gata6 P3 | agaacctggactgcgcttt | tttgctgctccctcaatgta | |
Gata6 P5 | cctggtgtcccaacacacta | tggccttgaattcactccat | |
Gapdh pmt | gggttcctataaatacggactgc | ctggcactgcacaagaagat | |
Gata4 | ggaagacaccccaatctcg | catggccccacaattgac | qRT-PCR |
Gata6 | ggtctctacagcaagatgaatgg | tggcacaggacagtccaag | |
Dab2 | ccacctccacaaagtaccaaa | caagcaagtcgtttgctgaa | |
Hdac3 | ctctggtgaagggtttggaa | tgtccatgtctcatccctga | |
Gapdh | agcttgtcatcaacgggaag | tttgatgttagtggggtctcg | Reference gene |
β-Actin | ctctcttccagccatctttcat | tataggtggtttcgtggatgc | |
Tbp | gggagaatcatggaccagaa | gatgggaattccaggagtca | |
Ppib | tcttcataaccacagtcaagacc | accttccgtaccacatccat | |
Hprt | cctcctcagaccgctttt | cctggttcatcatcgctaatc | |
Ppar | ccttccctgtgaactgacg | ccacagagcgctaagctgt |