Figure 3

ChIP analysis of histone modifications at Gata factor G6P1 and G4P1 promoter loci in EBs in relation to maternal diet. (A) The Gata4 gene 5’ region is illustrated with three regions 5’ of exon 1 amplified by G4P1, G4P3 and G4P5. The Gata6 gene 5’ region is illustrated with three regions amplified by G6P1, G6P3 and G6P5. (B, C) ChIP analysis was performed using antibodies to H3Ac, H4Ac, H3K4Me3, H3K4Me2, H3K9Me3 H3K27Me3 and RNA Polymerase II and quantified by real-time PCR amplifying the G6P1 (B) and G4P1 (C) loci with results presented as fold enrichment over IgG. Duplicates of ChIP experiments were performed throughout for verification. Values are means from 6 cell lines from each diet group with standard errors represented by vertical bars, *p < 0.05, # < 0.1.