TY - JOUR AU - Sun, Congshan AU - Denisenko, Oleg AU - Sheth, Bhavwanti AU - Cox, Andy AU - Lucas, Emma S. AU - Smyth, Neil R. AU - Fleming, Tom P. PY - 2015 DA - 2015/01/21 TI - Epigenetic regulation of histone modifications and Gata6 gene expression induced by maternal diet in mouse embryoid bodies in a model of developmental programming JO - BMC Developmental Biology SP - 3 VL - 15 IS - 1 AB - Dietary interventions during pregnancy alter offspring fitness. We have shown mouse maternal low protein diet fed exclusively for the preimplantation period (Emb-LPD) before return to normal protein diet (NPD) for the rest of gestation, is sufficient to cause adult offspring cardiovascular and metabolic disease. Moreover, Emb-LPD blastocysts sense altered nutrition within the uterus and activate compensatory cellular responses including stimulated endocytosis within extra-embryonic trophectoderm and primitive endoderm (PE) lineages to protect fetal growth rate. However, these responses associate with later disease. Here, we investigate epigenetic mechanisms underlying nutritional programming of PE that may contribute to its altered phenotype, stabilised during subsequent development. We use embryonic stem (ES) cell lines established previously from Emb-LPD and NPD blastocysts that were differentiated into embryoid bodies (EBs) with outer PE-like layer. SN - 1471-213X UR - https://doi.org/10.1186/s12861-015-0053-1 DO - 10.1186/s12861-015-0053-1 ID - Sun2015 ER -