Table 1 Summary of monoclonal antibodies generated from selected hybridoma cell lines
From: Novel monoclonal antibodies to study tissue regeneration in planarians
Parental hybridoma | Clone at DSHB | Isotype | Dilution factor | Concentration of DSHB supernatant tested (μg/ml) | Tissues labeled |
|---|---|---|---|---|---|
1D9 | E11 | IgM kappa | 500 | 34 | Blastema, brain primordia, neoblast progeny |
1H6 | E9 | IgG1 kappa | 1000 | 54 | Axonal projections in CNS and PNS |
2C4 | C2 | IgG1 kappa | 1000 | 26 | Blastema, neurons, intestinal cells, anterior cells |
2G3 (polyclonal) | N/A | N/A | Undiluted | N/A | Muscle fibers |
5B1 | E6 | IgM kappa | 1000 | 18 | Protonephria, ciliated structures |
5E12 | E3 | IgG1 kappa | 1000 | 35 | Blastema |
6C8 | A2 | IgG1 kappa | 1000 | 26 | Blastema, intestinal cells |
6G10 | 2C7 | IgG1 kappa | 1000 | 59 | Muscle fibers |