Figure 7

Smed-6C8 labels intestinal cells and cells near the regeneration blastema. (A-C) Whole-mount view of intact or regenerating planarians immunostained with 6C8 (green) and co-labeled with 6G10 (magenta) in panels B-D and/or counterstained with DAPI (blue) in D and F. (A) 6C8-labeled cells near or within the intestine. (B, C) 6C8 cells are located within the anterior (shown in B) and posterior (shown in C) intestinal branches or in contact with the enteric musculature wall, which is delineated with 6G10 labeling. Examples of cells observed outside the enteric muscle boundary are highlighted with arrowheads. (D) 6C8 labels cell nuclei (observed within the boundary of the enteric musculature). (E, F) 6C8⁺ cells appear near the anterior and posterior regeneration blastemas at 3 dpa and are detected in the blastema at all later timepoints assayed (examples highlighted with arrowheads). By 5 dpa, examples of 6C8⁺ cells were detected far from the blastemas (arrows). Higher magnification image of the anterior blastema shown in F. Yellow asterisks indicate non-specific labeling of secretory cells. Dashed boxes indicate the area of the high magnification image shown in the insets. Images are maximum intensity projections except for A and E. Anterior of is to the top in all images. Scale bars: (A, E) 200 μm; (B-D, F) 50 μm.