Overview of the basic anatomy of asexual
recognized by the monoclonal antibodies generated in this study and tissue fixation protocols tested. (A) Cartoon of the basic anatomy of asexual S. mediterranea with arrows highlighting some of the major organs labeled with the monoclonal antibodies generated in this study. PR, photoreceptors; Int, intestine; CG, cephalic ganglia; VNC, ventral nerve cords; Ph, pharynx. (B) Summary of the creation of the monoclonal antibodies used in the subsequent experiments. dpa: days post amputation. (C) A heat map summarizing the labeling efficiency for each antibody following eight variations of a formaldehyde-based fixation protocol or Carnoy’s fixation. For each fixation and antibody, at least 2 experiments were performed with ≥ 4 worms, which were scored independently by 2 or more individuals. The fixation protocols are named according to the reagents used for each processing step. HCl, hydrochloric acid; FA, formaldehyde; ProtK, Proteinase-K; NAC, N-Acetyl Cysteine; Me, methanol; Red, reduction solution.