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Table 1 Optimized ETC. conditions for various tissues

From: Improved application of the electrophoretic tissue clearing technology, CLARITY, to intact solid organs including brain, pancreas, liver, kidney, lung, and intestine

Organ Optimized ETC. time (days) Constant current (mA) Varying voltage (V) Replacement interval of clearing solution (days)
Brain Whole brain: 12-16 280 22 ~ 26 5
3-mm-thick-block: 3 N/A
Pancreas 8-12 250 7
Kidney 18-22 280 7
Liver 18-22 280 7
Intestine 8-12 250 7
Lung 13-17 250 7
  1. n = 30 for optimization of each organ clearing. ETC., electrophoretic tissue clearing; mA, milliampere; V, voltage; N/A, not available.