Pancreatic vessel imaging in the intact adult mouse pancreas. In adult mouse tissues (12 weeks old), imaging was performed after CLARITY. (A) Three-dimensional (3D) projection (left panel) and rendering (right panel) of clarified mouse pancreas without capillary immunostained for α-smooth muscle actin (green). Scale bar, 300 μm (Additional files 3 and 4). (B) Three-dimensional projection (left panel) clarified mouse pancreas with capillary immunostained for α-smooth muscle actin (green). Scale bar, 200 μm. Merged image with manually traced (green, middle panel) and 3D rendering extracted capillary (right panel). Representative data were chosen from five independent experiments.