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Table 3 Effects of knockdown for mud on binucleation and asymmetric cell division (asym. cell div.)

From: Isoform-specific functions of Mud/NuMA mediate binucleation of Drosophilamale accessory gland cells

RNAi target

Frequencies of phenotypes in mitotic behaviors during

binucleation

asym. cell div.

Central spindle assembly

Contractile ring formation

Cell rounding

Abnormal spindle orientation***

Multi-bristle

None (fng-Gal4)

0% (N = 35, 6)

0% (N = 35, 6)

5% (N = 40, 6)

8% (N = 40, 6)

0% (NW = 60)

(0%, partial*)

(3%, partial**)

mud.IR

80% (N = 35, 7)

86% (N = 35, 7)

28% (N = 40, 5)

35% (N = 40, 5)

85% (NW = 40)

(20%, partial*)

(14%, partial**)

mud S .IR + Dcr2

27% (N = 37, 5)

29% (N = 37, 5)

5% (N = 42, 5)

31% (N = 42, 5)

0% (NW = 40)

(51%, partial*)

(41%, partial**)

  1. Numbers of cells and tissues (pairs of lobes) observed are shown as (N = cells, tissues).
  2. Numbers of adult wings observed are shown as (NW = wings).
  3. *A phenotype class with partial assembly of central spindle-like structure.
  4. **A phenotype class with partial accumulation of F-actin accompanied by furrow progression.
  5. ***Spindle orientated horizontally (0° ± 45°) rather than vertically in metaphase.