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Table 3 Effects of knockdown for mud on binucleation and asymmetric cell division (asym. cell div.)

From: Isoform-specific functions of Mud/NuMA mediate binucleation of Drosophilamale accessory gland cells

RNAi target Frequencies of phenotypes in mitotic behaviors during
binucleation asym. cell div.
Central spindle assembly Contractile ring formation Cell rounding Abnormal spindle orientation*** Multi-bristle
None (fng-Gal4) 0% (N = 35, 6) 0% (N = 35, 6) 5% (N = 40, 6) 8% (N = 40, 6) 0% (NW = 60)
(0%, partial*) (3%, partial**)
mud.IR 80% (N = 35, 7) 86% (N = 35, 7) 28% (N = 40, 5) 35% (N = 40, 5) 85% (NW = 40)
(20%, partial*) (14%, partial**)
mud S .IR + Dcr2 27% (N = 37, 5) 29% (N = 37, 5) 5% (N = 42, 5) 31% (N = 42, 5) 0% (NW = 40)
(51%, partial*) (41%, partial**)
  1. Numbers of cells and tissues (pairs of lobes) observed are shown as (N = cells, tissues).
  2. Numbers of adult wings observed are shown as (NW = wings).
  3. *A phenotype class with partial assembly of central spindle-like structure.
  4. **A phenotype class with partial accumulation of F-actin accompanied by furrow progression.
  5. ***Spindle orientated horizontally (0° ± 45°) rather than vertically in metaphase.