Skip to main content

Table 2 Ability of Mud variants to rescue binucleation defects in mud mutants

From: Isoform-specific functions of Mud/NuMA mediate binucleation of Drosophilamale accessory gland cells

Genotype Frequencies of phenotypes in mitotic behavior during binucleation
Central spindle assembly Contractile ring formation Cell rounding Abnormal spindle orientation***
Wild-type (control) 0% (N = 42, 7) 0% (N = 42, 7) 0% (N = 49, 5) 6% (N = 49, 5)
(5%, partial*) (5%, partial**)
mud 4 hemizygote (mud 4/Y) + rescue construct (RC) No RC 82% (N = 40, 9) 87% (N = 40, 9) 30% (N = 40, 8) 40% (N = 40, 8)
(15%, partial*) (13%, partial**)
RC = DC281 0% (N = 30, 4) 0% (N = 30, 4) 8% (N = 38, 4) 5% (N = 38, 4)
(40%, partial*) (57%, partial**)
RC = mud PBD 63% (N = 30, 4) 67% (N = 30, 4) 37% (N = 30, 4) 17% (N = 30, 4)
(33%, partial*) (33%, partial*)
RC = mud L 30% (N = 43, 9) 30% (N = 43, 9) 12% (N = 41, 7) 44% (N = 41, 7)
(51%, partial*) (56%, partial**)
RC = mud S 13% (N = 40, 8) 18% (N = 40, 8) 34% (N = 44, 7) 20% (N = 44, 7)
(45%, partial*) (33%, partial**)
  1. Numbers of cells and tissues (pairs of lobes) observed are shown as (N = cells, tissues).
  2. *A phenotype class with partial assembly of central spindle-like structure.
  3. **A phenotype class with partial accumulation of F-actin accompanied by furrow progression.
  4. ***Spindle orientated horizontally (0° ± 45°) rather than vertically in metaphase.
  5. DC281: Chromosomal duplication encompassing the mud gene region.