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Table 1 Effect of various genetic manipulations on central spindle assembly and contractile ring formation during binucleation

From: Isoform-specific functions of Mud/NuMA mediate binucleation of Drosophilamale accessory gland cells

Manipulation Genotype Frequencies of phenotypes in mitotic behavior
Central spindle assembly Contractile ring formation
None (wild-type) Canton-S 0% (N = 42, 7) 0% (N = 42, 7)
(5%, partial*) (5%, partial**)
Induction of cell rounding fng-Gal4 (Tub-Gal80 TS) > sterile 20-like kinase 0% (N = 34, 7) 0% (N = 34, 7)
(12%, partial*) (18%, partial**)
fng-Gal4 (Tub-Gal80 TS) > shotgun.IR 0% (N = 37, 6) 0% (N = 37, 6)
(8%, partial*) (14%, partial**)
Disruption of centrosome*** centrosomin HK21 homozygote 6% (N = 36, 6) 6% (N = 36, 6)
(11%, partial*) (8%, partial**)
Sas-4 S2214 homozygote 9% (N = 34, 7) 9% (N = 34, 7)
(18%, partial*) (29%, partial**)
Activation of contractile ring formation fng-Gal4 (Tub-Gal80 TS) > pebble 0% (N = 34, 6) 44% (N = 34, 6)
(56%, partial*) (41%, partial**)
fng-Gal4 (Tub-Gal80 TS) > sqh D20.D21 6% (N = 33, 6) 39% (N = 33, 6)
(64%, partial*) (48%, partial**)
sqh-GFP 11% (N = 36, 6) 14% (N = 36, 6)
(47%, partial*) (53%, partial**)
Septin-2-GFP 3% (N = 36, 7) 14% (N = 36, 7)
(22%, partial*) (42%, partial**)
  1. Numbers of cells and tissues (pairs of lobes) observed are shown as (N = cells, tissues).
  2. *A phenotype class with partial assembly of central spindle-like structure.
  3. **A phenotype class with partial accumulation of F-actin accompanied by furrow progression.
  4. ***Very low frequency of cells shows obvious misorientation of spindle axis.