Table 1 Effect of various genetic manipulations on central spindle assembly and contractile ring formation during binucleation

None (wild-type)

Canton-S

0% (N = 42, 7)

0% (N = 42, 7)

(5%, partial*)

(5%, partial**)

Induction of cell rounding

fng-Gal4 (Tub-Gal80 ^TS) > sterile 20-like kinase

0% (N = 34, 7)

0% (N = 34, 7)

(12%, partial*)

(18%, partial**)

fng-Gal4 (Tub-Gal80 ^TS) > shotgun.IR

0% (N = 37, 6)

0% (N = 37, 6)

(8%, partial*)

(14%, partial**)

Disruption of centrosome***

centrosomin ^HK21 homozygote

6% (N = 36, 6)

6% (N = 36, 6)

(11%, partial*)

(8%, partial**)

Sas-4 ^S2214 homozygote

9% (N = 34, 7)

9% (N = 34, 7)

(18%, partial*)

(29%, partial**)

Activation of contractile ring formation

fng-Gal4 (Tub-Gal80 ^TS) > pebble

0% (N = 34, 6)

44% (N = 34, 6)

(56%, partial*)

(41%, partial**)

fng-Gal4 (Tub-Gal80 ^TS) > sqh ^D20.D21

6% (N = 33, 6)

39% (N = 33, 6)

(64%, partial*)

(48%, partial**)

sqh-GFP

11% (N = 36, 6)

14% (N = 36, 6)

(47%, partial*)

(53%, partial**)

Septin-2-GFP

3% (N = 36, 7)

14% (N = 36, 7)

(22%, partial*)

(42%, partial**)