Figure 6
From: Isoform-specific functions of Mud/NuMA mediate binucleation of Drosophilamale accessory gland cells

Three types of mud splicing isoforms differently regulate morphologies of binucleating cells. (A–J) Rescue of mud mutant phenotypes by each mud isoform. Cross-sectional views of main cells in metaphase (A, D and G), late anaphase (B, E and H) and telophase (C, F, I and J) during the binucleation stage in mutants hemizygous for mud 4 with overexpression of FLAG::mud PBD(A–C), FLAG::mud L(D–F) or FLAG::mud S(G–J). Cells are labeled with phalloidin (magenta), anti-α-Tub antibody (green) and anti-P-H3 antibody (blue). The cell in (I) shows neither a central spindle assembly nor furrow progression. The cell in (J) shows furrow progression but no central spindle assembly. Arrowheads in (C, F, I and J) indicate equatorial planes. Scale bar in (A), 5 μm, is applicable to (A–J). (A’–J’) Schematic diagrams of (A–J). Cross-sectional views of mud S- knockdown main cells in metaphase (K), late anaphase (L) and telophase (M) during the binucleation stage. Cells are labeled with phalloidin (magenta), anti-α-Tub antibody (green) and anti-P-H3 antibody (blue). Arrowhead in (M) indicates an equatorial plane. Scale bar in (A), 5 μm, is applicable to (K–M). (K’–M’) Schematic diagrams of (K–M). Effects on cell morphologies in each of the three rescued genotypes ((A–C): mud 4 hemizygotes rescued by mud PBD, (D–F): mud 4 hemizygotes rescued by mud L, (G–J): mud 4 hemizygote rescued by mud S) and in mud S-knockdown cells (K–M) are listed under each set of diagrams.