mud Sis not required for the spindle orientation during asymmetric cell division. (A) Schematic diagram of molecular structures of human NuMA1 and Drosophila Mud. Shared domains are as indicated at the bottom. (B) Amino acid sequence alignment of the C-terminal regions of NuMA1-s and MudS. The blue box indicates short isoform- specific regions in NuMA1-s (amino acids 1701–1763) and MudS (amino acids 1880–1933). Red, orange and yellow overlays indicate similarities as indicated at the bottom. (C) Schematic diagram of SOP lineage in Drosophila wing margin. Cells indicated at the bottom right are produced in this lineage. Mud forms a complex with Gα and Pins that is localized asymmetrically (red crescent). (D–F) Bristles on adult anterior wing margin in wild-type (D), in knockdown of all mud isoforms (E), and in knockdown specific to mud S(F). Arrowheads in (E) indicate multi-bristle phenotypes.