Skip to main content

Table 2 Optimization of whole animal sample processing

From: Generation of cell type-specific monoclonal antibodies for the planarian and optimization of sample processing for immunolabeling

mAb Tissue specificity Fixation protocol
    Mg Mg Mg
HCl HCl NAc HCl HCl NAc
Mcn FA FA Mcn FA FA
2G4 Intestine & epidermis + + + ++ + +
3F11 None (MeOH bleach) - - - - - -
3F11 Intestine (PBS bleach) - - - - + +
3G9 Intestine - + + ++ + +
4D2 Intestine + - - ++ + +
1E12 Nuclei, elevated in epidermis and intestine + + - + - -
1H8 Nuclei, elevated in CNS - - + - + +
2C11 Peripharyngeal secretory cells and processes - + + - + ++
2D2 Subset of neurons and processes - + ++ - + ++
2H3 Muscles ++ + ++ ++ + ++
3G7 Epidermal basement membrane - + - - + -
3H3 Epidermis, enriched in cell:cell junctions ++ + + ++ - -
  1. Qualitative assessment of signal:noise for the predominant tissue labeled by each mAb. “-“ indicates negligible or non-uniform labeling, poor morphology, and/or high background. “+“ indicates moderate specificity, uniform labeling, and/or robust labeling accompanied by moderate background. “++” indicates robust signal with minimal noise.