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Table 1 Monoclonal antibodies, isotypes, and tissue specificity

From: Generation of cell type-specific monoclonal antibodies for the planarian and optimization of sample processing for immunolabeling

Clone

Isotype*

Tissue(s) recognized

1A10

IgM

Intestine

1B11

IgM

Intestine†

1C3

IgM

Intestine†

1E12

IgM

Epidermal & intestinal nuclei

1F12

IgM

CNS neurons (small subset)

CNS processes in neuropil (subset)

PNS processes in neuropil (subset)

1H8

IgM

Nuclei (elevated in CNS)

1H11

IgM

Cilia†

2C11

IgG1

Peripharyngeal secretory cells & projections

2D2

IgM

CNS neurons (small subset)

CNS processes in neuropil (subset)

PNS processes in neuropil (subset)

2E8

IgM

Pigment cups†

2G4

IgM

Intestine & epidermis

2H3

IgM

Muscles

3A4

IgM

Intestine†

3A10

IgM

Intestine†

3D4

IgM

Marginal/anterior secretory cells intestine (weak)†

3D10

IgM

Ventral puncta†

3F11

IgG1

Intestine

3G7

IgM

Epidermal basement membrane

3G9

IgM

Intestine

3H3

IgM

Epidermis, enriched in cell:cell junctions

4D2

IgM

Intestine

4H4

IgM

Ventral secretory cells

4H8

IgM

Epidermal nuclei (subset)†

  1. *All mAbs possessed kappa light chains.
  2. †Pattern based on primary screen; mAb has not been characterized further.