Figure 2
From: TGF-β signaling can act from multiple tissues to regulate C. elegansbody size
Design and construction of pharyngeal sma-3 minigene rescue constructs. (A) Expression of the sma-3p::sma-3::gfp translational fusion. Expression is visible in the nuclei and cytoplasm of hypodermal cells (h), intestinal cells (i), pharyngeal muscles (pm) and pharyngeal marginal cells (mc). (B) The F47B7.7 (marg-1) transcriptional reporter is strongly expressed in pharyngeal marginal cells. (C) Top, a genomic BglII (B) - SalI (S) sma-3 fragment was cloned into a similarly digested sma-3 cDNA clone to create the sma-3 “minigene” (below) used for rescue experiments, under the control of various promoters (arrow); see text for details. (D) Pharyngeal expression of the sma-3 minigene carrying an in-frame N-terminal GFP tag under the control of the myo-2 and marg-1 promoters. Expression is absent from pharyngeal gland cells (arrowhead) and weak expression is occasionally observed outside of the pharynx (arrow).