Expression of maternally derived KHDC3, NLRP5, OOEP and TLE6is associated with oocyte developmental competence in the ovine species

Table 2 Total RNA content of ovine Ad and Pr oocytes and embryos

Stage	Mean concentration (pg/μl)	Mean SE (pg/μl)	Mean RIN value	Isolated RNA (pg) per oocyte or embryo*	Estimated RNA (pg) per oocyte or embryo**
GV Ad	314	41.6	8.3	471	942
GV Pr	170	61	5.9	253	538
MII Ad	95	53	6.7	142	285
MII Pr	80	22	5	119	238
2 cell Ad	58	6	3.5	86	185
2 cell Pr	80	14	4.4	120	240

RNA samples isolated from pools of 10 oocytes/embryos were analysed with Agilent 2100 Bioanalyzer (Agilent Technologies) with an RNA 6000 Pico kit (Agilent Technologies), specifically designed for analysis of low concentrated RNA samples (analysis range of total RNA: 50–5000 pg/μl). RNA samples isolated from 4- 8- and 16- cell stage embryos are not included because below the lower limit of analysis (<50 pg/μl).
*Mean quantity of total RNA isolated per oocyte or embryo.
**Estimated RNA per oocyte or embryo, taking into account the RNA loss during RNA isolation, as resulted from analysis of the exogenous luciferase mRNA.

ISSN: 1471-213X