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Table 1 Primers used for real-time PCR experiments

From: Expression of maternally derived KHDC3, NLRP5, OOEP and TLE6is associated with oocyte developmental competence in the ovine species

Gene

GenBank accession n.

Primers

Annealing temperature

Size (bps)

KHDC3

XM_004011872

F: 5' CAGACCCTGCTTCACGTTCA 3'

60°C

150

R: 5' CTTCTCAGAGCTTCGCGCC 3'

NLRP5

HM037368.1

F: 5' CAGCCTCCAGGAGTTCTTTG 3'

59°C

212

R: 5' GACAGCCTAGGAGGGTTTCC 3'

OOEP 1

KF218578

F: 5' ATCCGCTGGTGTTCTTCCTG 3'

60°C

149

R: 5' GAACACGGTGACTTCGACCA 3'

OOEP 2

KF741040

F: 5' TCCCCAAACTCCTTGCAGTG 3'

60°C

114

R: 5' CGGCAGGTAGGTGTCTGAAT 3'

TLE6

XM_004009373

F: 5' TACCTGCGCACCTGCCTGCT 3'

58°C

195

R: 5' ATTGGTGAAGCCAGCAAAAG 3'

ACTB

NM_001009784

F: 5' TTCCTGGGTATGGATCCTG 3'

60°C

162

R: 5' GGTGATCTCCTTCTGCATCC 3'

luciferase

AF093685

F: 5' GCTGGGCGTTAATCAGAGAG 3'

58°C

151

R: 5' GTGTTCGTCTTCGTCCCAGT 3'