Figure 2

Dnajb13 mRNA and DNAJB13 protein at different developmental periods of mouse testis. (A) Quantitative RT-PCR for Dnajb13 from different periods of mouse testis. The expression of Dnajb13 mRNA was detected as early as postnatal week 1, and developmentally up-regulated to postnatal week 3, then was constant. After postnatal week 4, its expression was constant at a high level. Each bar of the histogram represents the mean (±SEM) of at least three experiments. (B) WB of DNAJB13 from different periods of mouse testis. The beta-ACTIN was also detected as a control. DNAJB13 protein was undetectable until postnatal week 4. There was an apparent delay in DNAJB13 protein translation.