Figure 5
From: 14-3-3 epsilon prevents G2/M transition of fertilized mouse eggs by binding with CDC25B
Co-localization of exogenously expressed 14-3-3ε and CDC25B. A: GFP-CDC25B and RFP-14-3-3ε were co-localized primarily in the cytoplasm at early G2 phase in two groups (25/30 and 26/30 eggs, respectively). B: GFP-CDC25B-S321A translocated to the nucleus (24/30 eggs) whereas GFP-CDC25B-WT were detected in the cytoplasm co-localized with 14-3-3ε (25/30 eggs) at late G2 phase in fertilized mouse egg. 14-3-3ε localized predominantly in the cytoplasm at late G2 phase in CDC25B-S321A group. Bar =20 um.