Figure 4
From: 14-3-3 epsilon prevents G2/M transition of fertilized mouse eggs by binding with CDC25B
Co-localization of endogenous 14-3-3ε and CDC25B in fertilized eggs. The representative cells were fixed, permeabilized and immunolabeled for confocal microscopy at different phases. A, B: The CDC25B signals and 14-3-3ε signals were co-localized primarily in the cytoplasm at G1and S phases, respectively. C: The CDC25B signals and 14-3-3ε signals were observed in the cytoplasm at early G2 phase. D: The 14-3-3ε signals were detected in the cytoplasm, but nuclear accumulation of CDC25B signals were observed at late G2 phase. E, F: The CDC25B signals and 14-3-3ε signals distributed in the cytoplasm again at early M phase and late M phase, respectively. G: Negative control showed no signal of CDC25B and 14-3-3ε. H: CDC25B was localized in the cytoplasm 15 h after control siRNA microinjection at early G2 phase. I: The CDC25B signals moved to the nucleus from cytoplasm 15 h after 14-3-3ε siRNA injection at early G2 phase. Bar =20 um.