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Figure 3 | BMC Developmental Biology

Figure 3

From: In vitroculture and somatic cell nuclear transfer affect imprinting of SNRPN gene in pre- and post-implantation stages of development in cattle

Figure 3

Maternal expression analysis of SNRPN gene at day40. Average percentages of maternal expression in tissues (placenta and fetus tissues: brain, heart, liver and muscle) obtained from embryos produced by AI (black bar) and IVF (open bars) and SCNT (gray bars). SNRPN reverse transcription-polymerase chain reaction fragments were cloned into a plasmid vector and sequenced for the parental SNP. Ratios were based on the total number of paternal alleles (G; Bos indicus SNP) found over the total number individual clones sequenced. a, b subscripts represent significant differences within tissues (P < 0.05).

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