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Figure 1 | BMC Developmental Biology

Figure 1

From: In vitroculture and somatic cell nuclear transfer affect imprinting of SNRPN gene in pre- and post-implantation stages of development in cattle

Figure 1

Identification of an allele-specific single nucleotide polymorphism (SNP) on SNRPN exon 2. Schematic representation of the bicistronic SNURF-SNRPN locus in Bos indicus and Bos taurus, depicting the progression from DNA to protein. Genomic: Numbered boxes represent exons respective to SNURF (black) and SNRPN (gray); hatched box represents the DMR analyzed. Primer positions and amplicon size for DMR and allele-specific cDNA are indicated above. mRNA: Single transcript with the SNURF (black) and SNRPN (gray) open reading frames. Bos taurus and Bos indicus cDNA sequences are aligned to indicate the SNP (G to A transition; open box) at position +151 (GeneBank AF1010140). Protein: Amino acid alignment of SNURF protein is shown for Bos taurus, Bos indicus and compared to a consensus sequence. Differences from the consensus are indicated in white boxes. The Bos indicus SNP leads to a change from an aspergine to a serine in the phosphorylation site for casein kinase II (CK2) (adapted from [19]).

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BMC Developmental Biology

ISSN: 1471-213X

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