CDC2/SPDY transiently associates with endoplasmic reticulum exit sites during oocyte maturation

Table 1 Listing of markers used to establish the identity of cortical structures in immature oocytes.

Name	Type	Target	Reference	Present in a cortical structure
CDC2	mouse monoclonal antibody	Cell division cycle 2, catalytic subunit of MPF	[22]	+
PSTAIR	mouse monoclonal antibody	Cyclin binding domain in all Cdks	[23]	+
SEC23	goat polyclonal antibody	SEC23, component of the coat protein II complex	[18]	+
P-GM130	rabbit polyclonal antibody	GM130 phosphorylated on serine 25	[27]	+
Calnexin	rabbit polyclonal antibody	Calnexin, transmembrane protein	[26]	-
Gamma-tubulin	rabbit polyclonal antibody	Gamma-tubulin, cytoskeletal component	[25]	-
Mitotracker*	Fixable live cell dye	Oxidation within mitochondria	[28]	-
NUP153	mouse monoclonal antibody	Nucleoporin 153 kDa	[29]	-
GM130	mouse monoclonal antibody	Structural Golgi protein	[27]	-
Cyclin B	mouse monoclonal antibody	Cyclin B, regulatory subunit of MPF	[22]	-
SPDY	rabbit polyclonal antibody	SPDY, regulatory protein of CDC2	[19]	+

ISSN: 1471-213X