Figure 4

Gamma-tubulin, mitochondria, and NUP153 do not associate with ERES. (A-C) 0 h GV stage oocyte labeled for CDC2 (A, green), DNA (A, blue), and gamma-tubulin (B, red). The CDC2-labeled ERES cluster did not label with gamma-tubulin (C). (D-F) 0 h GV stage oocyte labeled for CDC2 (D, green), DNA (D, blue), and mitochondria, using the mitochondrial marker mitotracker (E, red). Mitotracker did not label CDC2-positive ERES (F). (G-I) Confocal section through the same oocyte as in D-F showing the GV. Note that the much lower staining intensity in G-I compared to D-F is solely the result of a ~65 μm change in depth of imaging, since acquisition settings and image enhancement were identical between these images. (J) 0 h GV stage oocyte labeled for NUP153 (green) and DNA (blue). None of the oocytes examined (0/15) showed a cortical domain. The 3 separate double lines around the GV are the result of small size and shape changes between the 3 consecutive sections used to produce the image. Images are either Z-projections of 3 consecutive sections (A-C, J), or single sections (D-I); scale bars represent 20 μm in C, F, I, and J. Arrows indicate CDC2-labeled ERES, and arrowheads denote the position of the GV.