Figure 3

The fragmented Golgi marker P-GM130 colocalizes with CDC2 at ERES in immature oocytes. (A-C) 0 h GV stage oocyte labeled for CDC2 (A, green), DNA (A, blue), and phosphorylated GM130 (B, red). Colocalization (yellow) of CDC2 and phosphorylated GM130 in a cortical compartment in the ooplasm is evident in the merged image (C). (D-F) 0 h GV stage oocyte from the same experiment as A-C, labeled with moIgG (D, green), DNA-marker (D, blue), and rabbit IgG (raIgG, E, red). None of the aspecific dots (examples are marked with white circles in D, F and yellow circles in E, F) colocalize in the merged image (F). This control image was subjected to the same enhancements as A-C, and selected to show confocal sections from an equivalent acquisition depth. (G-I) 0 h GV stage oocyte labeled for CDC2 (G, green), DNA (G, blue), and the ER-marker calnexin (H, red). No colocalization (yellow) of CDC2 and calnexin was observed in the merged image (I). Images are Z-projections of 6 consecutive sections (A-F), or 3 consecutive sections (G-I); scale bars represent 20 μm in C, F, and I, and 5 μm in C' and I'. Arrows indicate the region of the oocyte that is shown enlarged in the insets (A'-C' and G'-I'). Arrowheads denote the position of the GV.