Figure 5

3'UTR orthologs from zebrafish and Xenopus contain similar sequence elements but different architectures. (A) Schematic diagram of the zebrafish and Xenopus cyclin B1 3'UTRs depicting the RNA sequence elements that affect maturation-specific poly (A) addition and translation. The hexanucleotide sequences AAUAAA or AUUAAA, PBE: pumilio binding elements UGUA(N)AUA, CPE: cytoplasmic polyadenylation elements UUUUAU, UUUUUCAU, UUUUAAU, UUUUACU. The activity of the cyclin B1 3'UTRs in directing poly (A) addition and activating translation during oocyte maturation in both zebrafish and Xenopus (this study) are summarized on the right. (B) Schematic diagram comparing the 3'UTR architecture of zebrafish and Xenopus mRNAs. These 3'UTRs were chosen for comparison because previous studies demonstrated that the Xenopus 3'UTR in each pair is sufficient to direct poly (A) addition and activate translation during oocyte maturation. The following zebrafish mRNAs were used for analysis (listed as gene name, gene symbol, Accession number) cyclin A1, ccna1, accession BC095579; cyclin B1, ccnb1, NM_131513; b-actin2, bactin2, BC045879; cyclin B2, ccnb2, BC116569; wee1, wee1, BC116569; c-mos, mos, NM_205580, PRE – polyadenylation response element.