Figure 5

Ipiwi1 mRNA expression and cell proliferation (BrdU) in controls (A, B), during hydroxyurea treatment (C-J), after irradiation (K-N), and during starvation (O-R). (C) Upon three days HU treatment ipiwi1 mRNA could not be detected in neoblasts but was still present in testes (t) and ovaries (ov). (E) At five days of HU treatment ipiwi1 mRNA was present in all cells of the ovaries (ov) but not in testes (t, bracket indicates region of testes). (G) After 10 days ipiwi1 mRNA remained only in mature eggs; (t, testes; bracket indicates region of testes) (G). After 15 days no ipiwi1 mRNA could be detected (I) The number of S-phase cells strongly decreased during hydroxyurea treatment (D, F, H) and only single S-phase cells were found after 15 days (J). Irradiation (K-N) resulted in a complete elimination of ipiwi1 expression (K, M) and a strong reduction in the number of S-phase cells after one day (L) and one week (N) post irradiation. During starvation (O-R), ipiwi1 expression and protein localization were weakly reduced after one week (O, P). After five weeks of food deprivation, dramatic degrowth led to a strong reduction of Ipiwi1 mRNA and BrdU (Q, R). In all figures, anterior is to the left. t, testes; o, ovaries. Asterisk marks autofluorescence of diatoms within the gut. Scale bars 100 μm.