Figure 3

Ipiwi1 mRNA expression (A-G) and protein localization (A'-G') during posterior regeneration. One hour after cutting (A, A'), ipiwi1 expression could not be detected at the regeneration site. After 10 hours, ipiwi1 was upregulated below the epidermis (arrows) (B, B'). At 25 hours postamputation (C, C') a significant proportion of cells within the regeneration blastema were ipiwi1 positive. From 48 hours onwards ipiwi1 expression and protein were present in the differentiating genital blastema (open arrows in D-F'). (G, G') After 76 h, ipiwi1 expression reached default levels. Scale bars 50 μm.