Skip to main content
Figure 5 | BMC Developmental Biology

Figure 5

From: Mechanism of primitive duct formation in the pancreas and submandibular glands: a role for SDF-1

Figure 5

SDF-1 signaling controls pancreatic branching morphogenesis. Immunofluorescence analyzis of pancreatic tissue stained for E-cadherin, mucin-1 and DNA. (A) The dorsal pancreatic bud was dissected from e12.5 embryos and either fixed and processed for immunostainings (= e12.5 = day 0) or cultured on filter for 7 days (= day 7) prior to immunostaining analyzis. Dissected mouse e12.5 pancreas before the culture (Day 0) appears as a mass of epithelial cells (red; E-cadherin-positive) surrounded by mesenchyme (E-cadherin-negative). Some cells accumulate mucin-1 at their apical pole, but no lumina are formed. After 7 days in culture (Day 7), the epithelial mass has been remodeled and cells are polarized and form monolayers delineating lumina. (B) e12.5 pancreatic explants dissected from wild-type mouse and cultured for 7 days. Control and SDF-1 (300 ng/ml) treated explants form polarized monolayers delineating lumina and imbricated with mesenchyme. In contrast, treatment of the pancreatic explants with 20 μM AMD3100, a specific pharmacological inhibitor of CXCR4, inhibits epithelial morphogenesis, as seen by the maintenance of the epithelial cells in clusters. Scale bars, 50 μm.

Back to article page