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Figure 7 | BMC Developmental Biology

Figure 7

From: RFX2 is a candidate downstream amplifier of A-MYB regulation in mouse spermatogenesis

Figure 7

Location of A-MYB and B-MYB expression in mouse testis by immunofluorescence. (A-D). Green, B-MYB, pseudocolor red, DAPI). (A). Tubule of approximately Stage VI, in which B-spermatogonia (B) or early spermatocytes are the most B-MYB positive cells in the outer most germ cell layer. Nuclei of pachytene cells (P) are faintly positive. (B) Tubule of approximately Stage VIII. Leptotene spermatocytes (L) are strongly B-MYB positive, while mid-late pachytene spermatocytes (P) are unreactive. (C). Tubule of approximately stage X. Leptotene-zygotene spermatocytes (L-Z) are strongly B-MYB positive, while late pachytene spermatocytes (lP) are unstained. (D). Tubule of approximately stage I. Early pachytene spermatocytes (eP) remain positive for B-MYB. Spermatids are uniformly negative in these tubules. (E-G). A-MYB. (E). Tubule of approximately stage I. Nuclei of early round spermatids are A-MYB positive (arrowhead), while early pachytene spermatocytes and other cells in the outermost germ cell layer are uniformly negative. (F). Tubule of approximately stage VIII. Nuclei of late pachytene spermatocytes (lP) are positive while reaction of late round spermatid nuclei is reduced (arrowhead). (G). Tubule of stage XII. Nuclei of diplotene spermatocytes (D) and the condensed chromosomes of the metaphase of meiosis I (M) are A-MYB positive. Note that cells in the outermost germ cell layer (spermatogonia and early spermatocytes) are uniformly negative in all tubules.

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