Figure 4

Features of the 5' regulatory region of the mouse Rfx2 gene. (A). Nucleotide sequence with +1 based on [NCBI RefSeq:NM_009056]. Solid circles above nucleotides designate additional transcriptional start sites identified by 5'-RACE cloning [16]. Exon 2 is shown until the translational start. MYB binding sites (MBS) are indicated by tan shading. An NFY motif is indicated by blue shading. Perfect 9-nt GC boxes are shaded yellow. RFX half sites are boxed without shading. (B). The three Rfx2 MBSs are compared to the consensus and to the unusually strong chicken mim-1-A site. The sites are numbered starting closest to the transcriptional start site and shown as the A-rich strand, regardless of the actual orientation. MBS1 consists of partially overlapping sequences in opposite orientations designated 1A and 1B. (C). Diagram of the promoter region showing locations of factor binding sites. M, MBS; Sp, GC box; X, RFX half site. Amplified promoter fragments used in reporter vectors are indicated below (PFA, -274 to +24; PFB -274 to -132; PFC, -224 to -157).