Figure 4
From: FGF signaling controls caudal hindbrain specification through Ras-ERK1/2 pathway
Pea3 and MKP3 expression are FGF-dependent. Embryos were explanted and cultured during 2 h in control medium (a, c) or medium supplemented with 25 μM SU5402 (b, d) and assayed for expression of cMKP3 or cPea3. Embryos were electroporated with mFgf3 (e-g) or mvHnf1 (h), incubated during 3 h, 6 h or 8 h and then analyzed for cMKP3 (e-h) and for anti-GFP (g). Electroporated side is the right one. (g) Merge of MKP3 in situ hybridization and anti-GFP staining to show the extension of the electroporation and the overlapping of GFP and ectopic MKP3. All the pictures show whole-mount embryos with anterior to the top.