Figure 1
From: FGF signaling controls caudal hindbrain specification through Ras-ERK1/2 pathway
Fgf3 is rapidly induced after mvHnf1 overexpression. Embryos were electroporated with mvHnf1-GFP construct and incubated during different time periods. After the desired incubation period the hindbrain tissue was processed for total RNA extraction and one-step RT-PCR amplification for cFgf3 and mvHnf1. (A) Amplified bands corresponding to cFgf3 and mvHnf1 checked after 25 and 27 cycles. Samples correspond to 15 min (a), 30 min (b), 1 h (c) and 6 h (d) after mvHnf1 overexpression, or 6 h after mvHnf1-Q136E overexpression (e). Amplification of GAPDH was run in parallel to normalize sample values (f). (B) The ratio of intensity between the cFgf3 and the mvHnf1 band at each time point was calculated and plotted in the graphic, in order to analyze the relative level of cFgf3 expression. A quantification program was used to calculate the intensity of each band and it was expressed in percentage of volume of the band (cFgf3/mvHnf1, see M&M). (C) Comparison of cFgf3/mvHnf1 ratio after mvHnf1 or mvHnf1-Q136E overexpression.