Figure 3

The scrib/dlg mutant FCs at the posterior rarely adopt a default AFC fate. Wild type (A, C and E) and dlg^m52 (B) or scrib² (D and F) mosaic chambers stained for nuclei (DAPI, blue) and Slbo (red in A and B) or β-gal (red in C-F). The mutant clones are marked by lack of the nuclear GFP (green). (A, B) In stage 8 wild type egg chamber Slbo is exclusively expressed in the border cells at the anterior pole (A, A'). This border cell marker is not ectopically expressed in the dlg^m52 clone cells at the posterior (B, B'). (C, D) In the wild type, MA33 specifically labels the stretched cells, which cover the nurse cells at the anterior of the stage 10 egg chamber (C, C'). In the mosaic egg chamber, expression of this enhancer trap marker is present in the wild type stretched cells, but absent in scrib² FCs at the posterior (D, D') as the same as the wild type. (E, F) The enhancer trap insertion BB127 is specifically expressed in the centripetal cells in stage 10b wild type egg chamber (E, E'), and occasionally labels the scrib² follicle cells at the posterior (F, F'). Note that only one or two mutant cells adopt BB127- expressing cell fate (arrows in F, F').