Lineage tracing after OV ablation. (A, C, E) The location of the ablation and subsequent DiI placement is shown in an image of an OV (outlined in black) with an ablation (outlined in white; A', B', C'). The anterior (a), posterior (p) and the distal (d) and proximal (pr) axes are indicated. (A-B) A posterior ablation of the OV with the DiI placed on the dorsal edge near the distal tip led to a small peripheral dorsal region containing DiI labelled cells at E7. (C-D) Placement of the DiI more proximally toward the posterior edge of the ablation led to a large region of lineage traced cells spread over to distinct areas. (E-F) DiI placed on the dorsal distal edge of a distal tip ablation lead to a small region of retina containing lineage traced cells. Anterior (A), Posterior (P), Proximal (PR), and Distal (D) are indicated for the OV. Dorsal (D), Ventral (V), Nasal (N) and Temporal (T) are indicated for the retinal flatmounts. Scale bar = 1 mm.