Location of lineage traced cells after DiI placement. (A', C', E') Images of optic vesicles (OV) (outlined with a dashed line) show the location of each DiI placement. (A-B) DiI placements in the anterior OV result in lineage traced cells being observed in the nasal retina at both E7 (n = 6; A) and E17.5 (n = 6; B). (C-D) There was no overlap with the visinin free spot (circle) at E7 or the rod free zone (circle) at E17.5. Posterior DiI placements lead to DiI positive lineage traced cells in the temporal retina at E7 (n = 3; C) and E17.5 (n = 3; D). There was no overlap with the visinin free spot (circle) at E7 or the rod free zone (circle) at E17.5. (E-F) Anterior dorsal placements of DiI lead to lineage traced cells in the visinin free spot at E7 (n = 4; E) and the rod free area centralis at E17.5 (n = 4; F). Anterior (A), Posterior (P), Proximal (PR), and Distal (D) are indicated for the OV. Dorsal (D), Ventral (V), Nasal (N) and Temporal (T) are indicated for the retinal flatmounts. Scale bar = 1 mm.