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Figure 1 | BMC Developmental Biology

Figure 1

From: MidExDB: A database of DrosophilaCNS midline cell gene expression

Figure 1

CNS midline gene expression. (A) In situ hybridization (AP histochemistry) of the Poly-glutamine tract binding protein 1 (PQBP-1) gene at stage 15 of embryonic development showing prominent midline cell expression. Ventral view; anterior left. (B) Sagittal view of a single segment of a sim-Gal4 UAS-tau-GFP embryo at stage 17, in which tau-GFP is expressed in all midline cells and facilitates midline cell identification by confocal microscopy. The embryo was immunostained with: (1) anti-GFP (green) to visualize the cytoplasm of all midline cells and (2) anti-Engrailed (red), which stains the nuclei of ventral unpaired median interneurons (iVUMs; yellow arrow points to 1 iVUM), median neuroblast (MNB), and MNB neuronal progeny (arrowhead points to 1 MNB progeny). The embryo was also hybridized to a probe for pale (blue) which stains the H-cell (white arrow). Dorsal top; anterior left.

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