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Figure 4 | BMC Developmental Biology

Figure 4

From: Smad4-dependent pathways control basement membrane deposition and endodermal cell migration at early stages of mouse development

Figure 4

Increased expression of basement membrane proteins by Smad4 mutant EB outgrowths. A. Day 4 wild-type and Smad4 null EBs grown for 2 days on fibronectin-coated (25 μg/ml) dishes. Smad4 mutant EBs express increased levels of laminin. B. Cryosections of day 4, day 7 and day 10 suspension EBs stained for collagen IV. Smad4 mutant EBs display progressively increased basement membrane deposition beneath the outer endoderm layer.

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