Figure 6

ras^KP-induced apoptosis is correlated with JNK signaling. ras^KP-induced cell death could be suppressed by using RNA interference (RNAi) to down regulate the endogenous expression of DTRAF1 (A), dTAK1 (B), Hep (C), Eiger(E), and Wengen (F). GMR-driven expression of a dominant-negative form of JNK (Bsk^DN) also partly suppresses the ras^KPeye phenotype (D). Immunostaining eye discs with an anti-phosphorylated JNK antibody reveals an increased level of activated JNK signaling in ras^KPmutant (H) as compared to wild-type (G). Genotypes: (A) GMR-Gal4/UAS-DTRAF1-IR; ras^KP/ras^KP, (B) GMR-Gal4/CyO; UAS-dTAK-IR, ras^KP/ras^KP, (C)GMR-Gal4/CyO; UAS-Hep-IR, ras^KP/ras^KP, (D) GMR-Gal4/CyO; UAS-BskDN, ras^KP/ras^KP, (E) GMR-Gal4/UAS-Eiger-IR; ras^KP/ras^KP, (F) GMR-Gal4/UAS-Wengen-IR; ras^KP/ras^KP, (G) wild-type, (H) ras^KP/ras^KP.