Figure 5

JNK and Hid cooperate in ras^KP-induced cell death. The reduction of the JNK signaling activity and inhibiting Hid activity by either overexpressing p35 or using the hid null do not have an effect in the wild-type background (A, B, C). However, the reduction of both activities lead to a strong suppression of the ras^KPapoptotic phenotype (D, E, F). When p35 or Hep-IR is expressed under the GMR promoter in the ras^KPmutant eye disc, apoptosis still occurs as detected by acridine orange staining (G and H, respectively). However, when both JNK pathway and Hid activity are inhibited in the ras^KPmutant, very few dying cells could be detected (I). Genotypes: (A) GMR-Gal4/GMR-p35; UAS-DTRAF1-IR, (B) GMR-Gal4/GMR-p35; UAS-Hep-IR, (C) GMR-Gal4/Cyo; UAS-Hep-IR, Df(3L)H99/hid⁰⁵⁰¹⁴, (D) GMR-Gal4/GMR-p35; UAS-DTRAF1-IR, ras^KP/ras^KP, (E)GMR-Gal4/GMR-p35; UAS-Hep-IR, ras^KP/ras^KP, (F) GMR-Gal4/Cyo; UAS-Hep-IR, Df(3L)H99, ras^KP/hid⁰⁵⁰¹⁴ras^KP. (G) GMR-Gal4/UAS-p35; ras^KP/ras^KP. (H) GMR-Gal4/Cyo; UAS-Hep-IR, ras^KP/ras^KP. (I) GMR-Gal4/GMR-p35; UAS-Hep-IR, ras^KP/ras^KP.