Figure 6

ptc2 is expressed at the zebrafish CMZ. In-situ hybridization for the ptc2 transcript shows expression at the retinal margin at 56hpf (arrows in A). At 72hpf, transcript is detected throughout the CMZ (B) and at the iridio-corneal angle (green arrows), with high magnification of CMZ region (E) revealing more robust staining adjacent to the RPE and in a region just apposed to the GCL (red arrows). Whole mount images from a dorsal view of sense (C) and anti-sense (D) probes in-situ hybridization show that staining is specific.