Figure 3

Cilia presence correlates with Hh signaling capacity in urogenital sinus mesenchyme cells. UGSM-2 cells were plated at confluence or subconfluence and grown in 0.1% FBS (FBS-) or 10% FBS (FBS+) media. Following 48 hrs incubation +/- 1nM Shh peptide, RNA was isolated and gene expression was determined by Real Time RT-PCR. Data represents the mean ± standard error of three replicate experiments. * indicates p < 0.05.