Table 1 Primer sequences.
From: Gene expression profiles during early differentiation of mouse embryonic stem cells
A) Marker/Housekeeping genes (note: Brachyury, Beta-actin, Hprt and Gapdh also came up in the arrays) | ||
|---|---|---|
Gene | Primer sequence | PCR fragment size (bp) |
Oct4 F | GAGCACGAGTGGAAAGCAAC | 521 |
Oct4 R | CGCCGGTTACAGAACCATAC | Â |
NANOG F | TTACAAGGGTCTGCTACTGAGATG | 431 |
NANOG R | GCAATGGATGCTGGGATACT | Â |
18Smm F | GTAACCCGTTGAACCCCATT | 150 |
18Smm R | CCATCCAATCGGTAGTAGCG | Â |
GscF | CAGATGCTGCCCTACATGAAC | 157 |
GscR | TCTGGGTACTTCGTCTCCTGG | Â |
Fgf5 F | TGTGTCTCAGGGGATTGTAGG | 136 |
Fgf5 R | AGCTGTTTTCTTGGAATCTCTCC | Â |
KDR F | TTTGGCAAATACAACCCTTCAGA | 112 |
KDR R | GCAGAAGATACTGTCACCACC | Â |
Hprt F | CACGGACTAGAACACCTGC | 229 |
Hprt R | GCTGGTGAAAAGGACCTCT | Â |
Brachyury F | CATGTACTCTTTCTTGCTGG | 312 |
Brachyury R | GGTCTCGGGAAAGCAGTGGC | Â |
GATA4 F | CCCTACCCAGCCTACATGG | 138 |
GATA4 R | ACATATCGAGATTGGGGTGTCT | Â |
Rex1 F | CGTGTAACATACACCATCCG | 128 |
Rex1 R | GAAATCCTCTTCCAGAATGG | Â |
Nestin F | CCGCTTCCGCTGGGTCACTGT | 227 |
Nestin R | CTGAGCAGCTGGTTCTGCTCCT | Â |
NodalF | TTCAAGCCTGTTGGGCTCTAC | 162 |
Nodal R | TCCGGTCACGTCCACATCTT | Â |
GapdhF: | ACCACAGTCCATGCCATCAC | 432 |
Gapdh R: | TCCACCACCCTGTTGCTGTA | Â |
B-actinF: | CGTGGGCCGCCCTAGGCACCA | 242 |
B-actin R: | TTGGCCTTAGGGTTCAGGGGG | Â |
B) Primers, Confirmed and interesting genes: | ||
GSE8766 | Â | Â |
Gene | Primer sequence | PCR fragment size (bp) |
Aur F | AAATTGAAAGGAATCAGACTAGA | 147 |
Aur R | GACCACTGTCTGTAACACCC | Â |
Atp5b F | CCTGCATGGAAGGAAACCTG | 238 |
Atp5b R | GTCACATGGGGAAGCTGGTG | Â |
HnrpkF: | CCCCAACCCTGTTTGTAAGG | 293 |
HnrpkR: | GGACCAGATACAGAACGCACA | Â |
Dppa5F: | TCGGAGACACAAGGACTGGA | 269 |
Dppa5R: | CCCACAGGGATCTCGAATGTC | Â |
SgceF: | TGTCACGGTATTTGGTTCTCAA | 170 |
SgceR: | CGCAGACTACAGGTAAATGGTA | Â |
Rplp1F: | ACCGAAGCCCATGTCATCTT | 211 |
Rplp1R: | CTTTCTGGCCTGGCTTGTTT | Â |
Ssr2F: | TGGTTGAGTTCGGGGTAAGA | 274 |
Ssr2R: | AGCGGGAGTTTGACAGGAGA | Â |
Hspa8F: | GGGTTGCAGACTTTCTCCAGT | 239 |
Hspa8R: | AAGGCTGAGGATGAGAAGCA | Â |
Eif4a1 F: | CATCCAGCAGCGAGCTATTC | 271 |
Eif4a1R: | CAGCTTCTGCACCTCAGCAC | Â |
Rpl8 F: | CTCCAAAGGGATGCTCCACA | 238 |
Rpl8R: | GCCACAGTCATCTCCCACAA | Â |
Hspca1F: | ATCTGCACCAGCCTGCAAAG | 176 |
Hspca1R: | AACTGGACTCGGGGAAGGAG | Â |
BG063704 F: | GAACTCCAGACCTCCAGACCA | 184 |
BG063704 R: | TTGCTTTGGGCAACAACTGA | Â |
Rps2F: | TACCTGTTCTCCCTGCCCATT | 180 |
Rps2R: | AACACCAAGACCAACGTGACC | Â |
BG069915F: | GGAGTATGGAACGACCCTCTCA | 201 |
BG069915R: | GAGCAGTGATTCTCAACCTTGC | Â |
BG067484F: | GCCTCGATCAGAAGGACTTG | 193 |
BG067484R: | GACCCGCTGAATTTAAGCAT | Â |
BG067621F: | GCTCCCAAGATCCAACTACGA | 257 |
BG067621R: | AGCCTGAGAAACGGCTACCA | Â |
Cox4i1F: | CGCAGTGAAGCCAATGAAGA | 246 |
Cox4i1R: | GCTTTCCCCACTTACGCTGA | Â |
Ftl1F: | GCTGCCTAGTGGCTTGAGAGG | 216 |
Ftl1R: | ATGGGCAACCATCTGACCAA | Â |
TktF: | TATGGACTGGCCCTCGCTAA | 286 |
TktR: | GGGAGCCACAGAGGTTGATG | Â |
BG063737F: | GACGAGCACACAGGGAAACC | 300 |
BG063737R; | GGAGAGAAGGAGGGGCAAGA | Â |
BG069482F: | CCCTCGGATACCTGATGCTG | 167 |
BG069482R: | TGAGAAATGACGGAGCCTTG | Â |
Tubb5F: | TGGGAGGTGATAAGCGATGAA | 257 |
Tubb5R: | GGCCTTTAGCCCAGTTGTTG | Â |
Rps28 F: | CAGGTGCGAGTGGAATTCATG | 198 |
Rps28 R: | TGCTTTATTTAACAGTTGCAGATCA | Â |
H3133B01F: | CAGCCATTCAGCAAAGGAGA | 283 |
H3133B01R: | TCTTGGGCAGGGTCTGTAGG | Â |
H3134D10 F: | GCTCGGCTGTGTCAAGATGAAG | 227 |
H3134D10 R: | CATGGGTCAGAACACCTTGCTT | Â |
GSE8625: | Â | Â |
Mki67F: | CCTTGGCTTAGGTTCACTGTCC | 250 |
Mki67R: | TGCAGAATCCAGATGATGGAGC | Â |
PterF; | CATGTCCCACCTTGACAGGAC | 245 |
PterR; | CCGTACTTCATCAACCGATGC | Â |
Scmh1F: | GGACCCAGTGTAGGAAGAGAGACC | 206 |
Scmh1R: | ATTGCTTCTGGCGTTTGGAC | Â |