Figure 3
From: Arginine methyltransferase CARM1/PRMT4 regulates endochondral ossification
CARM1 regulates Cyclin D1 gene expression. (A) Lysates of SW1353 cells transfected with wt or mutant Flag-Sox9 were immunoprecipitated with anti-Flag antibody. Precipitates were subjected to Western blotting with an anti-beta-catenin (middle panel) and anti-Flag antibody (lower panel), respectively. Interaction of beta-catenin with wt Sox9 was almost abolished in the presense of high-dose CARM1 (upper panel). (B) Left: Expression of Cyclin D1 mRNA (ISH) in the humerus of E16.5 heterozygous, mutant and E14.5 wt and Tg embryos. Right: Expression of Cyclin D1 mRNA from E16.5 limb buds by RT-PCR. (C) Transcriptional regulation of the Cyclin D1 promoter by Flag-Sox9 and pCMX-CARM1 expression plasmids. Activating of Tcf mt reporter was also measured by a luciferase assay. Statistical significance is assessed by one-way analysis of variance and unpaired Student's t-test (*).