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Figure 7 | BMC Developmental Biology

Figure 7

From: Selenoprotein N is dynamically expressed during mouse development and detected early in muscle precursors

Figure 7

Somitogenesis in wild-type and Sepn1-/- embryos. A: Whole mount in situ hybridization, and corresponding vibratome sections, against Myf5, MyoD, Scleraxis (E11.5) and TrkA (E13) performed on Sepn1+/+ and Sepn1-/- embryos. No difference was observed in somite organisation and size (black fragments) between wild-type and mutants embryos. The expression pattern of the tested factors appeared unaltered in absence of SelN. Vibratome sections correspond to the trunk level for Myf5 and to the limb bud for MyoD. B: Immunostaining against myosin heavy chain on E11.5 and E13 wild-type and deficient embryos. The expression patterns were identical between the embryos. Myotome is outlined with a white broken line. C: Western blot analysis against myogenic factors, performed on proteins from wild-type and deficient E12.5 whole embryos, revealed no alteration of their expression in the mutant embryos. α-tubulin was used for normalization. Scale bars = 400 μm. d: drg; nt: neural tube.

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