Figure 5

SelN deficiency in the murine model. Sepn1 expression analyzed by qRT-PCR (A) and Western blot (B) performed on whole E12.5 littermate embryos: wild-type (+/+), heterozygous (+/-) and homozygous mutants (-/-). 18s gene and α-tubulin were used for normalization. Sepn1 transcript expression was almost abolished in the homozygous mutants and SelN was undetectable by Western blot in these mice. *, p < 0.05.