Figure 7
From: YA is needed for proper nuclear organization to transition between meiosis and mitosis in Drosophila
Histone H3-FLAG distribution on chromatin of YA-deficient eggs and embryos. Immunostaining of 0–15 minute old laid unfertilized H3-FLAG; Ya2 eggs, and H3-FLAG;Ya2 embryos. DNA shown in red, H3-FLAG in green. A-D are projections of multiple confocal images, with insets used to show nuclei in a greatly different focal plane. (A) YA-deficient egg with polar body rosette with H3-FLAG staining. (B) YA-deficient egg with four female meiotic products all with H3-FLAG staining. (C) YA-deficient embryo with two apposed nuclei and a third nearer the egg cortex, all with H3-FLAG staining. (D) YA-deficient embryo with five nuclei, two of which are more rounded and have H3-FLAG staining while three others do not. The H3-FLAG-staining nucleus not in the inset is closer to the three unstained nuclei along the Z axis than it is to the other H3-FLAG-staining nucleus shown in the inset. Ya2 eggs n = 24, embryos n = 58. Bar = 10 μm.