Figure 6
From: YA is needed for proper nuclear organization to transition between meiosis and mitosis in Drosophila
PCNA distribution on chromatin of wildtype and YA-deficient eggs and embryos. Immunostaining of 0–15 minute old laid unfertilized wildtype and Ya2 eggs, and wildtype and Ya2 embryos. DNA shown in red, PCNA in green. A and B are projections of multiple confocal images. (A) Four decondensed wildtype egg nuclei with PCNA on DNA, likely in S phase. 55% of wildtype eggs had PCNA staining nuclei, all synchronous. (B) Variously condensed Ya2 egg meiotic products with PCNA staining on 2 out of 3 areas of chromatin. 26% of Ya2 eggs had PCNA-positive nuclei; 2% were synchronous with PCNA, 24% were asynchronous. (C) Embryo with five decondensed wildtype meiotic products with PCNA staining on all. Two apposed pronuclei (inset) and three meiotic products forming a polar body (only two are visible in this plane). (D) Ya2 embryo with two nuclei in two planes; one near the embryo cortex (inset) and one deeper in. Only one of the two has PCNA staining (bracket). Wildtype n = 42 eggs, n = 215 embryos. Ya2 n = 34 eggs, n = 168 embryos. Bar = 10 μm.